Rapid diagnosis of Argentine hemorrhagic fever by reverse transcriptase PCR-based assay
نویسندگان
چکیده
منابع مشابه
PCR - Based Reverse Transcriptase Assay
ES cells. This phenomenon has been observed in our laboratory in a number of cases. Clones putatively identified as homologous recombinants by Southern analysis can be quickly checked for random insertion of extra copies of the targeting vector by PCR. Given the small difference in size between the two alleles and use of only two primers common to both WT and mutant DNA, the amplification rate ...
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A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR assay for classical swine fever virus (CSFV) was developed and evaluated in experimentally infected swine. The assay detected CSFV, representing different phylogenetic groupings, but did not amplify viral RNA from related pestiviruses. The assay met or exceeded the sensitivity (1 to 100 50% tissue culture infective doses per ml) ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1995
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.33.5.1327-1332.1995